Application

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated phospho Histone H2A (Thr120) in 500 µg of colcemid treated HeLa acid extract.

Dot Blot (Specificity) Analysis: 0.5 µg/mL from a representative lot detected phospho Histone H2A (Thr120) and not non-phosphorylated Histone H2A peptide or other peptides corresponding to modified histones.

Research Sub CategoryHistones

Detect Histone H2A using this rabbit polyclonal antibody, Anti-phospho Histone H2A Antibody (Thr120) validated for use in western blotting, Dot Blot & IP.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H2A is one of four components of the core nucleosomal structure. The nucleosome represents a unit of chromatin in which DNA is wrapped around a histone octamer. Histones undergo a number of post-translational modifications (PTM) in response to various stimuli that may induce changes in the structure of the nucleosome and hide or expose DNA sequences. Histone H2A, in particular, undergoes acetylation on lysine 5 by the Tip60 enzyme which may promote unfolding of chromatin and transcription. In addition, H2A is phosphorylated on serine 1 and serine 139 (H2A.X) residues which mark mitosis and gene repression, and DNA damage, respectively. Gene repression may also result from the sumoylation of H2A's lysine 126 residue. In addition, ubiquitination of the H2A lysine 119 residue may play a role in spermatogenesis. It is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human Histone H2A phosphorylated at Thr120.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in untreated and colcemid treated HeLa acid extract.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected phospho Histone H2A (Thr120) in 10 µg of colcemid treated HeLa acid extract, and demonstrated a decrease in signal in untreated HeLa acid extract.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~16 kDa observed